In-use contamination of a hospital-grade disinfectant

Highlights

• An investigation documented contamination of an in-use hospital-grade disinfectant.
• The disinfectant contained 9.3 × 10⁴ CFU of S. marcescens and A. xylosoxidans.
• Serratia was 10²-fold less sensitive to fresh disinfectant than a control strain.
• Whole genome sequencing of Serratia revealed sdeXY, sdeAB, smfY and sugE-like genes.
• Contamination was due to failure to follow recommended disinfection protocols.