Abstract

Background

The source of transmission of Clostridioides difficile in healthcare institutions is frequently unknown. The aim of this prospective cohort study was to assess the association between strains cultured from patients and shoe soles of healthcare workers (HCW), as already shown in the operation theatre, but not on general hospital wards in an acute care institution.

Methods

We conducted a study at a university tertiary care center in Switzerland. From October 2019 to July 2020, shoe soles of HCW were cultured for C. difficile twice per shift while taking care of a patient infected with toxigenic C. difficile. Additional risk factors were assessed by interviewing involved HCW. Patients’ fecal samples were processed by routine microbiological methods. Similarity of the HCWs’ and patients’ strains was determined by whole genome sequencing (WGS).

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